Laboratory and statistical analyses continue on the studies described
under objectives 1 and 2. Under objective 2, a series of studies have been
completed to determine the effects of vitamins and isoflavones alone and in
combination on cell proliferation as measured by thymidine incorporation. In
this study, the isoflavones genistein, glycitein, and daidzein at 10 uM alone
all inhibit growth of PC-3 prostate cancer cells by 64, 36, and 37%,
respectively. Vitamins E, A, and D inhibited PC-3 cell growth by 95, 64, and
98%, respectively. Vitamin E in combination with genistein further inhibited
growth another 2% over Vitamin E alone. Vitamin D treatment exhibited some
negligible additive decreases in combination with genistein and glycitein. We
are awaiting final approval from the Institutional Animal Care and Use
Committee to perform the immunosuppressed mouse study described under
objective 3. The first study will examine the effects of vitamin E alone and
in combination with a diet high in soy isoflavones will be performed first
based on our in vitro studies of Objective 2. Under Objective 4, we are
beginning Real Time-PCR studies of how dietary vitamins and phytoestrogens
effect the expression of the peroxisome proliferator-activated receptor (PPAR)
a potential component of the transcriptional machinery regulated by these
bioactive compounds. Our research groups have found in another system that soy
diets rich in isoflavones regulate PPAR expression.
Research from this project was also highlighted in an article in "Perspectives"
magazine on Cancer Research at SIUC (See "inner
workings").
